The present EIA, which is superior to RIA in terms of convenience and disposal of waste materials, is expected to be practically useful to monitor intact digoxin in human serum. Mean digoxin concentrations measured in serum samples (n=42) from digoxin-treated patients by EIA using the new antiserum and the commercial anti-digoxin antiserum were 1.26 and 1.39 ng/ml, respectively. Recovery was within the range of 96.0-105.0%. Digibind is a sterile freeze-dried powder of antigen binding fragments (Fab) obtained from antidigoxin antibodies raised in sheep. Intra-assay and inter-assay variations were less than 9.3% and 8.8%, respectively. Compared with a commercial anti-digoxin antiserum clinically used to monitor digoxin concentration in human serum, our antiserum showed much higher specificity for intact digoxin. The developed enzyme immunoassay showed a quantification range of 0.2 to 10 ng/ml and exhibited high specificity for digoxin, with low levels of cross-reaction to dihydrodigoxin (12.3%), digitoxin (7.84%), digoxigenin bisdigitoxoside (0.31%), digoxigenin monodigitoxoside (0.19%), and digoxigenin (0.05%). Since we have other medical options, digitalis is not that frequently used these days-but we still do. In the present study, we aimed to establish an enzyme immunoassay (EIA) for measuring serum digoxin level in patients, using digoxin 3'-hemisuccinate-β-D-galactosidase as an enzyme-labeled antigen. The Digibind (digoxin immune fab).: Digitalis has been in used for many years.Started in ancient time (the foxglove plant)-and can useful in select patients. We previously developed a radioimmunoassay for digoxin using an antiserum raised against digoxin 3'-hemisuccinate-bovine serum albumin.
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